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mutant, suggesting that there is a really low volume of Xis-impartial 89K excision. qPCR Investigation revealed that xis

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locus and an extrachromosomal circular sort of 89K working with certain primers that face outward with the built-in island or anneal to chromosomal DNA flanking the insertion site (Fig. 2A). Primer combos P1/P2 and P3/P4 had been used to amplify the left and right junction locations respectively. Primer pair P2/P3 was made to detect the presence of a circular 89K, and primer pair P1/P4 was used to amplify the repaired chromosomal junction fashioned upon excision.

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Parallel conjugative mating experiments had been also performed To judge if the genes proposed for being involved in the excision and circularization of 89K also are involved with the transfer method. As proven in Table 1, an int

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